p6 buffer exchange columns Search Results


p 6 spin columns  (Bio-Rad)
96
Bio-Rad p 6 spin columns
P 6 Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p6 cells  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc p6 cells
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
P6 Cells, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p6 buffer exchange columns  (Bio-Rad)
96
Bio-Rad p6 buffer exchange columns
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
P6 Buffer Exchange Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad micro bio spin p 6 ssc column  (Bio-Rad)
96
Bio-Rad bio rad micro bio spin p 6 ssc column
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Bio Rad Micro Bio Spin P 6 Ssc Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micro bio spin p6 spin columns  (Bio-Rad)
96
Bio-Rad micro bio spin p6 spin columns
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Micro Bio Spin P6 Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio gel p 6 dg media  (Bio-Rad)
94
Bio-Rad bio gel p 6 dg media
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Bio Gel P 6 Dg Media, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin p 6 gel  (Bio-Rad)
98
Bio-Rad bio spin p 6 gel
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Bio Spin P 6 Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad spin mini columns  (Bio-Rad)
94
Bio-Rad bio rad spin mini columns
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Bio Rad Spin Mini Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin chromatography columns  (Bio-Rad)
96
Bio-Rad bio spin chromatography columns
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Bio Spin Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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centrifugal desalting column  (Bio-Rad)
95
Bio-Rad centrifugal desalting column
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Centrifugal Desalting Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p 6 spin column  (Bio-Rad)
96
Bio-Rad p 6 spin column
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
P 6 Spin Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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self packed buffer exchange column  (Bio-Rad)
97
Bio-Rad self packed buffer exchange column
Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat <t>P6</t> <t>cells</t> in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).
Self Packed Buffer Exchange Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat P6 cells in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).

Journal: Biomolecules

Article Title: Mechanistic Target of Rapamycin Complex 1 Signaling Links Hypoxia to Increased IGFBP-1 Phosphorylation in Primary Human Decidualized Endometrial Stromal Cells

doi: 10.3390/biom11091382

Figure Lengend Snippet: Hypoxia treatment inhibits IGF-1R activity in decidualized primary HESCs. CM was collected from decidualized primary HESCs treated under normoxic (N) (incubator air) or hypoxic (H) (1% O 2 ) conditions for 72 h and buffer exchanged with high glucose DMEM media. Equal total IGFBP-1 (50 ng) in the buffer exchanged media were incubated with recombinant human IGF-1 (1 ng) and used to treat P6 cells in culture. P6 cells were lysed and Western blotting was performed (equal loading, 30 µg protein) to determine changes in IGF-1-induced IGF-1R autophosphorylation (Tyr1135) compared to IGF-1 Control 1 (CTR-1). Media with IGFBP-1 (50 ng) from primary decidualized (HESCs) cells under normoxia was considered as Control 2 (CTR-2). The Western blot data are summarized in the bar graphs ( n = 4). Data was first normalized to total IGF-1R followed by β-actin. Values are given as mean + SEM. *, p < 0.05; ** or ## , p = 0.001–0.05 versus control as per one-way ANOVA. Tukey’s multiple comparisons test. * = significant difference compared to IGF-1 positive control (CTR-1); # = significant difference compared to conditioned sample control (CTR-2).

Article Snippet: The treatment was terminated by aspirating the P6 CM and, subsequently, attached P6 cells were lysed using cell lysis buffer (Cell Signaling Technologies) containing protease and phosphatase inhibitors (Sigma) as described earlier [ ].

Techniques: Activity Assay, Incubation, Recombinant, Western Blot, Control, Positive Control